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The recommended daily dose of glutathione is 250-500 mg, which is generally regarded as safe, with possible mild side effects like bloating or cramping

doi: 10.3390/cancers17182948 55 NovielloMManfrediFRuggieroEPeriniTOliveiraGCortesiFet al

For cell scratch assay, HaCaT cells (13 1e6) or human keratinocyte primary cultures (5 1e5) were seeded in six/twelve-well plates with Dulbeccos modified Eagles medium (DMEM) containing 5% heat-inactivated FBS or with dermal cell basal medium supplemented with keratinocyte growth kit components (obtained from ATCC, USA)

We recommend blocking free thiols with alkylating agents like NEM or IAM during protein extraction to stabilize the modification