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The contents of sucrose and fructose were analyzed by Tang (1999), as follows: Sucrose analysis: 400-L supernatant added to 200-L 2-M NaOH that was boiled for 5 min, and then added 3-ml 30% (v/v) HCl, 1-mL.1% m-dihydroxybenzene, which were 80C water bathed for 10 min, 480-nm colorimetric analysis

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MDA was elevated in the iron group but largely normalized levels in the amlodipine-treated group, corroborating GSH-GSSG results that oxidative stress was elevated in the iron group and was at near-normal levels in the amlodipine-treated hearts (Figure 6A)