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Cell line generation ex vivo Three tumors for each treatment group were excised, placed in Petri dishes with DMEM/F12 medium, cut into fragments with a sterile scalpel, and subjected to digestion with 1 mg/ml collagenase type IV (Sigma-Aldrich) for 30 min at 37 C under aseptic conditions

Antibiotic therapy BE provides the perfect environment for colonization by various microorganisms, as mucociliary clearance is impaired, facilitating rapid bacterial growth on the airways mucosal surface without tissue invasion

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