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After intravenous administration of fluorescein, enhancement of the gaps between epithelial cells (1), leakage of fluorescein into the bowel lumen (2), and cell shedding (3) can be directly visualized using a confocal probe equipped with a 488 nm laser ( ex vivo permeability ( Finally, mucosal impedance testing is another tool to directly evaluate mucosal integrity, although most studies are limited to the esophagus

The isolated pain component also should be evaluated in patients who report pain or other descriptors of discomfort such as pressure

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