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doi: 10.1186/s12944-018-0852-z 112

2.15 Real-time quantitative PCR Total RNA was extracted from aortic tissue and endothelial cells using TRIzol reagent (Takara Bio Inc., 9108), following the manufacturers instructions

Indeed, in AD models, the effects of mitochondrial ROS were found to be much smaller compared with those of NOX-produced ROS (Angelova and Abramov, 2018)