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doi: 10.1016/j.bcp.2020.114201 53 ChenL.TianX.ZhangL.WangW.HuP.MaZ.et al

As shown above, SnCE1 reacted with the SUMO1-/SUMO3-PA probes independent of the N-terminal truncation suggesting the CE-clan protease domain is essential and sufficient for the reaction (Fig

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This indicates that lysine-containing tags remain functional under the milder biotinylation activity of AirID, whereas TurboID requires lysine-free tags such as REC to avoid biotinylation-dependent signal loss