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Why myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) may kill you: disorders in the inflammatory and oxidative and nitrosative stress (IO&NS) pathways may explain cardiovascular disorders in ME/CFS

doi: 10.3390/metabo14110589 154

Each sample, in duplicate, was amplified as follows: one cycle of 95C for 1 minute for pre-incubation, followed by 40 cycles of 95C for 30 seconds, 60C for 1 min and 72C for 1 minute, with a subsequent melting curve analysis using Mx3000p (Stratagene, Agilent Technologies, CA, USA)