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Observation of SHGs granules by FEG-ESEM The SHGs specimens collected from the gut of adult female worms or from the aforementioned cell incubations were fixed with 2.5% glutaraldehyde-PBS buffer (pH 7.4) at 4 C for 1 h to 2 h, then rinsed and fixed with 1% to 4% osmium tetroxide at 4 C for 1 h to 2 h, and sequentially dehydrated in graded ethanol series

Prepare three density gradient solutions of 1.03, 1.04 and 1.06 g/ml (equivalent to 5.4%, 6.4% and 10.3%, w/v iodixanol) by diluting Optiprep with Solution B (see Note 2)

Gene expression was normalized to the mRNA expression level of -actin as an endogenous control, and fold changes were calculated between treatment and negative-treatment control samples