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Moreover, the affinity of Acs1 for acetate is reported to be several times greater than that of the constitutively expressed Acs2 isoform, suggesting that Acs1 is primarily responsible for acetyl-CoA synthesis during growth on non-fermentable carbon sources such as ethanol 26

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Renal cortical and medullary tissues were collected and stored in the RNA later solution (Thermo Fisher Scientific Inc.) until use

For general daily support, oral glutathione precursors (N-acetylcysteine, alpha lipoic acid) are reasonable and inexpensive