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SKU: 59092663149 · From chapuisstores.ch

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Description

King, head laboratory scientist

Cells were fixed in 4% (v/v) paraformaldehyde (PFA) (Pierce, 28906) in PBS for 20 min and permeabilised in 0.1% (v/v) Triton X-100 in PBS (Sigma-Aldrich), or in 0.1% (w/v) saponin (sigma-Aldrich) in PBS when labelling LAMP1 compartments, for 5 min followed by blocking with 2% (w/v) BSA (Sigma, 05482) in PBS for 30 min