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The integrated approach demonstrated in this paper opens a new window to better studied the H 2 O 2 generation and variation in complete cell culture medium in a straightforward and rapid manner, which can greatly help scientists to investigate H 2 O 2 related biomedical phenomena

Moreover, GPER activates several cellular signaling pathways, including the PI3K/AKT and MAPK pathways, which are integral to various metabolic processes [127]