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This leads to the cleavage and translocation of AIF from the mitochondrial intermembrane space to the nucleus, phosphorylation of the histone H2A family member H2AX, mitochondrial release of the death-inducing protein Smac/direct IAP-binding protein with low PI (DIABLO), and inhibition of the anti-apoptotic protein X-linked inhibitor of apoptosis (XIAP)

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The high selectivity of affinity chromatography is caused by allowing the desired molecule to interact with the stationary phase and be bound within the column in order to be separated from the undesired material which will not interact and elute first

But used on different days, they complement each other beautifully