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The LOD and limit of quantification (LOQ) were calculated mathematically by the relationship between the standard error () of the calibration curve and its slope (S) using the multiplier according to ICH [50]ICH Q2 (R1), Validation of analytical procedures: text and methodology, in Proceedings of the International Conference on Harmonization, Geneva, Switzerland, 2005

and the National Natural Science Foundation of China (Grant No

doi: 10.3389/fbioe.2026.1751293 Received 21 November 2025 Revised 11 January 2026 Accepted 18 February 2026 Published 12 March 2026 Volume 14 - 2026 Edited by Yaying Sun, Shanghai General Hospital, China Reviewed by Yushu Wang, Tufts University, United States Yibo Ma, Second Affiliated Hospital of Dalian Medical University, China Updates Copyright 2026 You, Liu, Zhang, Zheng, Dou, Yao, Li, Wang and Huang