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Granstein RD, Wagner JA, Stohl LL, Ding W (2015) Calcitonin gene-related peptide: key regulator of cutaneous immunity

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For the preparation of DCs from bone marrow cell cultures, the cells flushed from murine femurs and tibias are cultured in the standard RPMI/10% FCS in the presence of 10 ng/ml IL-4 and granulocyte-macrophage colony-stimulating factor for 6 days prior to the harvesting of loosely adherent and non-adherent cells (see ref