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In contrast, ApoE4 drives dysregulation of lipid metabolism, endothelial inflammation, and abnormal activation of extracellular matrix remodeling pathways, which directly leads to widespread downregulation of astrocytic end foot associated genes in the BBB [88, 91]

On day 7 post transfection, 6-well plates were coated with 10 g/mL poly-D-lysine for 1 h at 37 C then washed 3X in 1X PBS

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