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Disruptions caused by this imbalance can lead to abnormal cellular processes that impair sperm quality and reduce their fertilizing ability [4, 22]

The efficacy evidence for edaravone is primarily derived from clinical trials in East Asian populations, and its benefits have been less consistently replicated in Western studies and real-world settings outside of Japan

After 30 min, the lysate was clarified by centrifugation (1500 g ), filtered (0.22 m filter), aliquoted and the E1E2-containing lysates were stored at 80 C

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