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SKU: 67006152196
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Cancer 124(7):1415-1427, 2018

Knowing these genes are identified to be essential for MAP survival in natural host, a mouse model was employed to evaluate their role in MAP survival in this common laboratory animal

GSTP, glutathione- S -transferase

Determination of free thiol groups using Ellmans reagent (DTNB assay) To quantify accessible thiol groups, 200 l of purified Chi proteins (0.26 mg/ml) were incubated with 5 mM reducing agent (DTT or -mercaptoethanol) for 30 min at 37 C