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Non-specific protein binding was blocked with 5% non-fat milk in Tris-buffer for 30 min, followed by overnight incubation with primary antibodies obtained from Abcam (mitochondrial complexes I-V and loading control VDAC)

Another study shows that urate administration elevated its levels in the SOD1G85R-expressing Drosophila model of familial amyotrophic lateral sclerosis (fALS) by activating the Akt signaling pathway and the catalytic subunit of glutamate-cysteine ligase (64)