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In mouse models of cerebral or renal artery occlusion, pretreatment with UroA appeared to mitigate the effects of ischaemia, reducing the extent of tissue damage and restoring impaired autophagy (Reference Wang, Huang and Jin62, Reference Ahsan, Zheng and Wu63)

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After homogenization, the mixture was centrifuged at 1500 rpm for 10 min at 4 C, separating the tissue, which was subsequently utilized for both RNA and protein extraction