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Description

Using Qubit2.0 fluorometer (Life Technologies, USA) to quantify the purified libraries, and the results were validated using an Agilent 2100 Bioanalyzer (USA) to determine the insertion size and calculate the molar concentration

Schuster, I

mTOR phosphorylation at Ser2448 indicates downstream target engagement, while S6K1 and 4E-BP1 phosphorylation reflects mTORC1 complex activity

The supernatant was then collected and protein was assayed on 96-well plates by adding 3 L of standard and 150 L of protein assay dye reagent (Bio-Rad, Hercules, CA, USA) diluted 1:5 with distilled water