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Preparation of Tissue Homogenate Two brain tissue homogenates were prepared, one in cold buffer (phosphate buffer saline, pH 7.4) in the ratio (1:10 w/v ) for the assay of GPX4, IL-1, TfR1 and ferritin and another in (50 mM potassium phosphate, pH 7.5) in the ratio (1:10 w/v ) for the assay of MDA, using tissue homogenizer (WiseTis homogenizer HG-15D Witeg Labortechnik GmbH, Germany)

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Following strict storage guidelines helps preserve structural integrity

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