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Instead Grx2, a mitochondrial isoform, exhibits de-glutathionylation activity for peptides and proteins but with a catalytic activity 10-fold lower with respect to Grx1 ( S -glutathionylation can be catalyzed by glutathione- S -transferase P (GSTP)

(PubMed) Horigan G, McNulty H, Ward M, Strain JJ, Purvis J, Scott JM

The folate cycle and methionine cycle are two major components of this network required for regulating amino acid homeostasis, including the three amino acids that make up glutathione: cysteine, glycine, and glutamic acid

Phalloidin Atto 747N (Sigma-Aldrich) was used for direct visualization of F-actin