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Since the above results showed that GSSG is elevated intracellularly in response to P

Metzger BE, Gabbe SG, Buchanan TA, et al

Agaricus bisporus and Agaricus brasiliensis (1 6)--d-glucans show immunostimulatory activity on human THP-1 derived macrophages

Plasmid pGex4T1-GST-TEV-PHF19 (residues 531580) was transformed into Rosetta2 competent cells, plated on an LB agar plate with ampicillin and chloramphenicol, and incubated at 37 C overnight