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Each well was then washed with a washing buffer solution provided by the company and 50 L of streptavidin-HRP solution was added to each well and the plate incubated at room temperature for 30 min with gentle shaking on a plate shaker

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The researchers found that the inositol blend significantly improved insulin sensitivity, reduced ovarian volume, and improved menstrual regularity with an efficacy comparable to the pharmaceutical intervention, but with a drastically lower incidence of gastrointestinal side effects