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Description

When fully dissociated, cells were centrifuged (230 g , 3 min) and the cell pellet resuspended and diluted to approximately 2.5E06 cells/mL in Recovery TM Cell Culture Freezing Medium (Gibco, #12648-010)

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Steinbrecht S, Kammerer S, Kpper J-H (2019) HepG2 cells with recombinant cytochrome P450 enzyme overexpression: their use and limitation as in vitro liver model

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These systemic reactions are also typically short-lived

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10.1371/journal.pone.0007476 PLoS One 14 BedrosianT

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