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This is consistent with a mechanism where the R471-carboxylate interaction is required to couple substrate binding to transport

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Although a previous study found that SAC did not inhibit hepatic CYP2E1 protein expression nor CYP2E1 mRNA levels [47], in this study, SAC treatment was associated with significantly reduced immunohistochemical CYP2E1 expression in liver tissue compared to controls (Figure 6 and Figure 7)