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Mixed with 500 L 70% EtOH and spun at 3000 g for 30 s at +4 C in a MiniElute column, washed with 350 L RW1, on-column digestion for 15 min at room temperature, washed with 350 L RW1, 500 L RLT, followed by 500 L 80% EtOH and spun for 2 min

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Recent studies have highlighted the pivotal role of the endothelial glycocalyx in systemic inflammatory diseases such as sepsis and atherosclerosis, where its degradation correlates with microcirculatory failure and organ dysfunction [8, 10, 13]

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