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Results Real-time monitoring of cytosolic and mitochondrial NADPH in primary cultured human aortic endothelial cells To determine compartmentalized NADPH levels, we overexpressed an NADPH indicator, iNap1 in either cytosol (cyto-iNap1) or mitochondria (mito-iNap3) of primary cultured human aortic endothelial cells (HAECs)