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Weak inhibition by hydroxybenzoic acid was also found in both isozymes, only 15 and 9% activity was inhibited, respectively [34]

More importantly, western blotting experiments showed that caffeine treatment significantly decreased UV irradiation-induced SOD2 acetylation (Figure 3D)

336 ZhouB.LiuY.KahnM.AnnD

HT-1080 cells are cultured in DMEM containing 10% fetal bovine serum, 1% supplemented non-essential amino acids and 1% pen/strep mixture (Gibco) and maintained in a humidified environment at 37C with 5% CO 2 in a tissue culture incubator