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Aggressive mixing can: Introduce air bubbles that promote surface-area oxidation Mechanically denature peptide structure (particularly relevant for larger peptides like TB-500) Create foam that is difficult to pipette accurately For peptides that dissolve slowly, allow the vial to stand at room temperature for 5-10 minutes after initial mixing before assessing dissolution

352 A bisubstrate inhibitor comprises two covalently linked fragments, each binding to a substrate or cofactor element, potentially mimicking the ternary transition state of a bireactant-catalyzed reaction

This makes the toxin or drug water-soluble so it can then be excreted from the body via watery fluids such as bile or urine

10.1038/s41467-022-35716-1 3 AvrahamiD.KlochendlerA.DorY.GlaserB