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Pre-fixation was done with 2.5% glutaraldehyde phosphate (0.1 M, pH 7.4) overnight at 4 C

It is produced from specific nutrients: amino acids that form its structure (cysteine, glutamine & glycine), vitamins, and minerals that participate in complementary reactions during its synthesis process (vitamins B2, magnesium and selenium)

In the prefrontal cortex, increased TDO and KMO expression alongside reduced IDO and KAT shifts metabolism toward QA production, while cerebellar KYNA levels inversely correlate with symptom severity, linking KP dysfunction to 5-HT metabolism and clinical features [235]

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