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For protein extraction, worm pellets were homogenized via bead beating in non-denaturing lysis buffer (100 mM HEPES pH 7.4, 300 mM NaCl, 2 mM EDTA, 2% Triton X-100) supplemented with EDTA-free protease inhibitor and PhosSTOP phosphatase inhibitor cocktails (Cat

Inducing Mesoderm Induction Following DMSO Treatment of hiPSCs The first stages of kidney organoid differentiation require treatment with Wnt agonist (CHIR99021) and BMP inhibitor (Noggin)

Glutamate cysteine ligase and the age-related decline in cellular glutathione: The therapeutic potential of gamma-glutamylcysteine