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Western blot analyses For the in vitro studies, differentiated C2C12 cells were collected at the end of experiments by cell lysis in ice-cold RIPA buffer

We did not find a significant non-linear relationship between dose (g/day) (coefficients = 1.72, p = 0.154) and changes in FBG, but we found a significant non-linear relationship (coefficients = 7.45, p = 0.020) between duration of intervention and changes in FBG, It seems that the effective duration for decreasing of FBG is 50 weeks (Figures 4A, 5A)

When dopamine levels decline, due to the death of dopaminergic cells, these messages no longer reach their destination, and so the regions of the brain that control movement no longer function properly