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NE-PER nuclear and cytoplasmic extraction Cells and kidney tissue samples were collected, cut into small pieces, washed with PBS, and then homogenized with beads in certain volume of cytoplasmic extraction reagent (CER) I in NE-PER Nuclear and Cytoplasmic Extraction Reagents kit (ThermoFisher, Waltham, MA) according to the weight of kidneys

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12 Current challenges in glutathione detection and biomarker analysis Quantification of GSH and related biomarkers like oxidized glutathione (GSSG) and the GSH/GSSG ratio is both clinically and research-wise challenging, predominantly due to preanalytical instability, methodological diversity, and biological interpretation difficulty