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In the ischaemia/reperfusion setting, all mice received B-017 or the negative control peptide intracardially 5 min before and intraperitoneally on consecutive days 1, 3, 5, and 7 of reperfusion

Dose escalation typically follows the classic 3 + 3 design, starting from extremely low doses and gradually increasing to higher doses [130]

Moreover, spermidine inhibits histone acetyltransferase p300 (EP300), a protein that suppresses autophagy, thereby enabling the activation of autophagy-related genes (Atg) (Reference Madeo, Carmona-Gutierrez and Kepp18)