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Description

DAPI staining Nuclear morphological changes in HCT 116 cells treated with Methotrexate (MTX) 47 MTX-loaded solid lipid nanoparticles (MTX-SLNs), and Lactoferrin-conjugated MTX-loaded SLNs (MTX-Lf-SLNs) were evaluated through 4,6-Diamidino-2-phenylindole (DAPI) staining

As soon as I dropped them off, I headed back out and spent some time composing the image

Inflammasome stimulation To activate the NLRP3 inflammasome, cells were primed for 3 h with LPS (100 ng/mL) followed by addition of 2 M (BMDMs, peritoneal macrophages, PBMCs), 6M (THP-1 differentiated macrophages) nigericin (Sigma-Aldrich, N7143-5MG), 2 mM (peritoneal macrophages) ATP or 50 M (THP-1 differentiated macrophages) R837 (InvivoGen, tlrl-imqs) for 30-60 min