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A landmark 2012 study published in Behavioural Brain Research administered 0.04mg/kg subcutaneously to rats with scopolamine-induced cognitive deficits and observed complete reversal of spatial memory impairments within 48 hours

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BNIP3, BCL2 interacting protein 3

These data demonstrate that metformin alters metabolism, specifically inhibiting oxidative phosphorylation, and induces significant mitochondrial dysfunction in both HCT116 and SW837 cells, supporting its metabolic modulatory effects in CRC in vitro