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54 HuangC.-K.LaiK.-P.LuoJ.TsaiM.-Y.KangH.-Y.ChenY.et al (2013)

Further non-limiting examples of single polynucleotides comprising a guide sequence, a tracr mate sequence, and a tracr sequence are as follows (listed 5' to 3'), where "N" represents a base of a guide sequence, the first block of lower case letters represent the tracr mate sequence, and the second block of lower case letters represent the tracr sequence, and the final poly-T sequence represents the transcription terminator: (1) NNNNNNNNNNNNNNNNNNNNgtttttgtactctcaagatttaGAAAtaaatettgcagaagctacaaagataaggcttt catgccgaaatcaacaccctgtcattttatggcagggtgttttcgttatttaaTTTTTT