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Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark

This finding does not establish causality, but it is consistent with a model in which robust mitochondrial peptide signaling is a feature of successful aging rather than a peripheral correlate

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Further studies are required to determine how CaMKII, Rho GTPases, and associated GEFs and GAPs participate in spine formation/elimination under physiological learning conditions in vivo