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Weigh the heart tissue and add corresponding reagents, grind it into tissue homogenate, centrifuge at 12,000 rpm for 15 min, and take the supernatant

At this stage, we say the patient has overt or clinical hypothyroidism There is some debate as to the benefit of treating those with sub-clinical hypothyroidism in symptomatic patients with a high TSH, but normal T4 a trial of treatment may be suitable Hypothyroidism is typically treated with a daily dose of thyroxine 1.6 g/kg lean body weight daily