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Comparison of biotinylation activity of biotin ligases via western blot For transient transfection, 300,000 HEK293T cells were seeded in a 12-well plate in DMEM/FBS

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The majority of plasmatic GSH originates from the liver, and for this reason an impairment in hepatic GSH synthesis has a systemic impact on redox balance and oxidative stress (1, 6, 7, 11)