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2006;44:472477 Adams PC

After thawing, cells were resuspended in 83 mmol/L sucrose, 10 mmol/L HEPES, pH 7.2 and homogenized by 30 strokes using pestle B in a 2 mL Dounce homogenizer

Second, electron transport chain (ETC.) impairment involves dysfunction of complexes I-IV, leading to reduced electron transfer efficiency, decreased ATP generation, and increased electron leakage that promotes excessive reactive oxygen species (ROS) production (Li JY