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Cell proliferation, as a key process of cell number expansion, is an indispensable link in wound healing, which drives new cells to fill tissue defects in the wound area and lays the cytological basis for wound healing

Free Radic Biol Med (2021) 176:31221

Pull-down assay by FLAG-Sirt7 To a 150 L of solution of nucleosomes (0.05 M) and FLAG-Sirt7 (WT, DM, or CA) (0.17 ng/L) in buffer A (20 mM Tris (pH 8.0), 100 mM NaCl, 5 mM MgCl 2 , 1 mM DTT, 2.5% glycerol, 0.01% Triton X-100), 5 L of anti-FLAG M2 magnetic beads (Sigma, #M8823) in 50 L of buffer A was added

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