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To mitigate these concerns, we prioritized age-appropriate pediatric controls, used the adult dataset only for cell-type annotation, and performed sensitivity analyses using Asia-based external cohorts, which supported the directionality of key CD4 T cellrelated findings

To better understand the involvement of B or CD4 T-lymphocytes in disease, SOD1 G93A mice were bred with CD4 knockout (CD4 / ) mice lacking surface expression of CD4, but with unaltered myeloid cells and CD8 + T- and B-lymphocytes

Materials and methods Study design and participants This single-center, prospective, case-control study was conducted between May 1, 2024, and September 1, 2024, at the Rheumatology Outpatient Clinic of Recep Tayyip Erdoan University Training and Research Hospital