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Excessive ROS production or depletion of antioxidant capacity leading to heightened oxidative stress has been implicated in fibrotic development across multiple organs, including the heart, liver, and lungs [46]

Available online at: 83 NarendraS.VenkataramaniS.ShenG.WangJ.PasapulaV.LinY.et al

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After 5 min of incubation at room temperature in plain RPMI-1640 medium, 100 nM Calcein-AM (Sigma-Aldrich, Germany) was added, and cells were incubated for 10 min at 37 C