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For ferrous iron imaging, cells were stained with 1 M FerroOrange (Dojindo) or 1 M Lyso-RhoNox in HBSS and incubated for 30 min in 5% CO 2 at 37 C

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Building upon this core machinery, the glucosinolate pathway involves three additional enzymatic modules completing metabolic transformation: (1) CYP83A1 converts aliphatic aldoximes to thiohydroximates, (2) UGT74B1 mediates glycosylation of sulfated intermediates (requiring prior sulfation by SOT17/18), (3) the myrosinase-TGG1/2 system, in concert with epitope-specific proteins (ESPs), hydrolyzes stored glucosinolates into bioactive compounds like SFN upon tissue damage (41)