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[2] Symptoms of deficiency include fatigue, weakness, pale skin, shortness of breath, and neurological disturbances such as paraesthesia, ataxia, and cognitive changes

Samples were fixed in 10% buffered formalin overnight at 4C, dehydrated with increasing concentrations of ethanol, embedded in paraffin, cut into 5 m sections, and stained with hematoxylin and eosin (HE) or Massons Trichrome Stain Kit (Sigma-Aldrich)

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Elderly patients with tea and toast diets and chronic alcoholics are at especially high risk