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Coimmunoprecipitation (co-IP) assay and immunoblotting Striatum tissues and cells were lysed using RIPA lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1% Triton X-100, and 0.5% NP-40) containing protease and phosphatase inhibitors for 1 h on ice and sonicated 3 times using a Sonics VC750 ultrasonic processor

What If I Notice a Side Effect

As previously mentioned, GLUD1 stability is regulated by methylation and ubiquitination

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