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The absorbance was measured at 450 nm (with reference wavelength 630 nm), and BH4 concentrations were calculated according to the standard curve

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Silencing information regulator 1/Forkhead box O1-related signalling pathway Silencing information regulator 1 (SIRT1) is a key factor in helping cells defend against oxidative stress, and it plays an important role in maintaining the balance between bone formation and bone resorption [21, 58]

Western blotting showed that both SLC7A11 and GPX4, which activate inhibitory signals, were expressed at higher levels in the GSH-treated group than in the control group, similar to the expression pattern of the adipogenesis-related marker PPAR (Fig