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Signaling specificity in the Akt pathway in biology and disease

Cell culture Swiss 3T3 fibroblasts, obtained from American Type Culture Collection (ATCC CCL 92), were maintained at 37 in Dulbecco's modified Eagle's medium supplemented with 25 mM HEPES (pH 7.4), 10% (v/v) fetal bovine serum, 100 units/mL penicillin, and 100 mg/mL streptomycin

Its already well-established that very high doses of salicylates (250300 mg/kg) in your system can cause tinnitus

Studies have found that Gpx4 knockout in mice causes embryonic lethality, whereas conditional knockout of Gpx4 leads to dysfunctions of the immune system, liver, kidney, brain and other tissues and organs in mice[28]